Produção de Lipases com Atividade de Hidrólise por Aspergillus Utilizando Subprodutos Agroindustriais, Óleo de Soja e Glicerol

Christian Oliveira Reinehr; Luísa Bortoluzzi; Valquiria Quoos de Morais; Tatiana Moresco Smaniotto; Cindiele Karen Zen; Débora de Oliveira; Helen Treichel; Luciane Maria Colla

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Produção de Lipases com Atividade de Hidrólise por Aspergillus Utilizando Subprodutos Agroindustriais, Óleo de Soja e Glicerol

Christian Oliveira Reinehr, Luísa Bortoluzzi, Valquiria Quoos de Morais, Tatiana Moresco Smaniotto, Cindiele Karen Zen, Débora de Oliveira, Helen Treichel, Luciane Maria Colla

Resumo

Lipases são enzimas aplicáveis a inúmeros setores industriais, podendo ser obtidas de fontes microbianas, a partir de fermentação em estado sólido utilizando matrizes oriundas de resíduos agroindustriais. Este trabalho teve como objetivo produzir lipases com atividade de hidrólise pelo fungo aspergillus utilizando resíduos agroindustriais e estudar a influência da concentração do indutor e da umidade do processo conduzido via fermentação em estado sólido. Foram utilizados dois planejamentos experimentais em sequência, um fatorial completo 3² (com três fungos do gênero aspergillus e três indutores) e outro 2² com dois pontos centrais (com umidade variando entre 60 % e 70 % e concentração de indutor variando entre 0 % e 4 %). as maiores atividades de hidrólise foram obtidas com o fungo aspergillus niger o-4 e com o óleo de soja como indutor, sendo que tanto a umidade quanto a concentração de indutor apresentaram influência significativa na produção de lipases. As maiores atividades de hidrólise, em torno de 25 u/g, foram encontradas nos pontos centrais (65 % de umidade e 2 % de óleo de soja) para 6 dias de fermentação.

Palavras-chave

Aspergillus niger; Enzimas; Fermentação em Estado Sólido; Indutores; Resíduos

Texto completo:

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Revista Ciências Exatas e Naturais - RECEN. Universidade Estadual do Centro-Oeste - UNICENTRO/PR, BRASIL.

Licenciada sob uma Licença Creative Common

ISSN 2175-5620 ON LINE; 1518-0352 IMPRESSO

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Produção de Lipases com Atividade de Hidrólise por Aspergillus Utilizando Subprodutos Agroindustriais, Óleo de Soja e Glicerol

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