Atividade proteolítica e lipolítica de bactérias lácticas isoladas de salames artesanais Proteolytic and lipolytic activity of lactic acid bacteria isolated from artisanal sausages

Danielle Carpiné; João Luiz Andreotti Dagostin; Herta Stutz Dalla Santa; David Chacón Alvarez; Nelcindo Nascimento Terra; Osmar Roberto Dalla Santa

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Atividade proteolítica e lipolítica de bactérias lácticas isoladas de salames artesanais
Proteolytic and lipolytic activity of lactic acid bacteria isolated from artisanal sausages

Danielle Carpiné, João Luiz Andreotti Dagostin, Herta Stutz Dalla Santa, David Chacón Alvarez, Nelcindo Nascimento Terra, Osmar Roberto Dalla Santa

Resumo

Algumas cepas de bactérias lácticas de produtos cárneos fermentados sintetizam proteases e lipases. Estas enzimas contribuem para a formação do aroma e promovem alterações desejáveis na textura e flavour do produto. O objetivo desta pesquisa foi verificar a atividade proteolítica e lipolítica de 46 cepas de bactérias lácticas isoladas de salames obtidos por fermentação espontânea. A atividade proteolítica foi testada em dois meios contendo caseína e extrato de carne. Para os ensaios de verificação de lipólise também foram utilizados dois meios diferenciais usando o Ágar Base Tributirina, um com adição de Tributirina e o segundo meio com adição de banha de porco. A atividade enzimática foi mensurada pela formação de halos ao redor de colônias isoladas. Das cepas avaliadas mais de 80% apresentaram atividade proteolítica, enquanto que nenhuma das cepas apresentou atividade lipolítica.

Palavras-chave: bactérias láticas; proteólise; lipólise.

Abstract

Some strains of lactic acid bacteria in meat products have the ability to synthesize proteases and lipases, enzymes which contribute for the development of the flavor and promote desirable texture alterations of the product. The objective of this research was to verify the lipolytic and proteolytic activity of 46 lactic acid bacteria strains, isolated from fermented sausages obtained from spontaneous fermentation. The proteolytic activity was tested in different medium supplemented with casein and meat extract. For the lipolytic assays Tributyrin Agar Base supplemented with tributyrin and pork fats were both used. The enzymatic activity was measured by zone formation around the isolated colonies. Among the evaluated strains more than 80% demonstrated proteolytic activity; however none of the strains presented lipolytic activity.

Key words: lactic acid bacteria; proteolysis; lipolysis.

Palavras-chave

bactérias láticas; proteólise; lipólise.

Texto completo:

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AMBIÊNCIA

ISSN 1808-0251 (Print) - Ambiência ISSN 2175-9405 (Online)
QUALIS-CAPES 2017-2020: B4

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Atividade proteolítica e lipolítica de bactérias lácticas isoladas de salames artesanaisProteolytic and lipolytic activity of lactic acid bacteria isolated from artisanal sausages

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Atividade proteolítica e lipolítica de bactérias lácticas isoladas de salames artesanais
Proteolytic and lipolytic activity of lactic acid bacteria isolated from artisanal sausages