Salycilic acid application in postharvest cabbage leaves butter

Fabiana Chiamulera Borsatti, Sérgio Miguel Mazaro, Claudiane de Abreu Dias, Rita de Cássia Dosciatti Serrão da Rocha, Douglas Junior Bertoncelli

Abstract


Considering the scarcity of studies related to the postharvest of kales and the inexistence of evaluation with resistance inducers in the species, we aimed to assess the effect of salicylic acid on the postharvest of collard greens over the physiochemical and biochemical variables. The experiment was conducted at the Federal Technological University of Paraná – Dois Vizinhos Campus. We used five treatments with entirely randomized design. The treatments consisted of using solutions with four concentrations of salicylic acid (0.5; 1.0; 1.5; 2.0 mM) and the control (distilled water), with four replicates of eight leaves. After the harvest, selection and standardization of the samples, the bases of the collard greens leaves were immersed in solutions with different treatments, during 10 minutes. The leaves were maintained in a B.O.D. incubator., during 192 hours, at the temperature of 8°C. The variables used for assessing the experiment were the mass loss of fresh matter, rottenness, level of vitamin C, chlorophyll, total phenols, total proteins and activity of the enzymes peroxidase and phenylalanine ammonia lyase (FAL). The application of AS maintained the contents of protein and total phenols at higher levels, as well as interfered on the activities of the peroxidase and FAL. The treatments did not present effect over the mass loss of fresh matter, and the content of vitamin C, chlorophyll and rottenness.


Keywords


Brassica oleracea; greenery; senescence.

Full Text:

PDF

References


ADOLOFO LUTZ. Normas Análiticas do instituto Adolfo Lutz: V. 1, Métodos Químicos e Fisicos para Análises de Alimentos.3.ed. SãoPaulo:IMESP,1985.p,394-395.

ALTVORST, A.C.; BOVY, A.G.; The role of ethylene in the senescence of carnation flowers: a review. Plant Growth Regulation, New York, v. 16, p. 45-53, Jan. 1995.

BEZERRA, A. P. L.; VIEIRA, A. V.; VASCONCELOS, A. A.; ANDRADE, A. P. S.; INNECCO, R.; MATTOS, S. H. Desempenho de plântulas de couve (Brassica oleracea var. Acephala) tratadas com cera de carnaúba hidrolisada. Horticultura Brasileira, v. 23. p. 395, 2005.

BIELESKI, R.L.; TURNER, N.A.; Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chomatograghy. Analitycal Biochemistry. Orlando, v. 17, p. 278-293, 1966. DOI 10.1016/0003-2697(66)90206-5.

BRADFORD, M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analitycal Biochemistry, Orlando, v.72, p.248-254, 1976.DOI 10.1016/0003-2697(76)90527-3.

CAMPOS, A. D.; Considerações sobre indução de resistência a patógenos em plantas. Documentos / Embrapa. 28 p., Documentos, 264; ISSN 1516-8840. Embrapa Clima Temperado, Pelotas 2009.

CHET, I. Biotechnology in plant disease control. 373p. New York: Wiley-Liss, 1993.

FILGUEIRA, F. A. R.; Novo manual de olericultura: agrotecnologia moderna na produção e comercialização de hortaliças.Viçosa: UFV, 2003, 402 p.

GRÜNER, R.; STROMPEN, G.; PFITZNER, A.P.; PFITZNER, U.M. Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1apromoter. European Journal of Biochemistry, v.270, p.4876-4886, 2003.

HAMMERSCHMIDT, R.; MÉTRAUX, J. P.; VAN LOON, L. C. Inducing resistance: a summary of papers presented at the first international symposium on induced resistance to plant diseases, Carfu, May 2000. European Journal of Plant Pathology, Dordrecht, v. 107, p. 1-6, 2001.

IMRAN, H.; ZHANG, Y.; DU, G.; WANG, G.; ZHANG, J. Effect of Salicylic Acid (SA) on delaying fruit senescence of Huang Kum pear. Frontiers of Agriculture in China, v. 1, n. 4, p. 456-459. 2007. DOI 10.1007/s11703-007-0075-y.

JALALI, B.L.; BHARGAVA, S.; KAMBLE, A. Signal transduction and transcriptional regulation of plant defense responses. Journal of Phytopathology, v.154, p.65-74, 2006.

KESMANN, H. STAUB, T: HOFMANN, C: MAETZKE,T: HERZOG J: WARD E UKNES, S: RYALS, J Inducyion os systemic acquired resistece in plants by chemicals. Annual Review Phytopathology. p. 439-459. 1997. DOI: 10.1146/annurev. py. 32.090194.002255.

KUHN, O. J. Indução de resistência em feijoeiro (Phaseolus vulgaris) por acibenzolar-S-metil e Bacillus cereus: aspectos fisiológiocs, bioquímicos e parâmetros de crescimento e produção. Piracicaba, 2007, 140p. Tese de doutorado. Escola Superior de Agricultura. “Luiz de Queiroz”, Universidade de São Paulo.

JENNINGS, A. C.; The determination al dihydroxy phenolic compounds in extracts of plant tissues. Analitycal Biochemistry. Orlando, v. 118, p. 396-398, 1991.

MATSUNO, H.; URITANI, I. Physiological behavior of peroxidase isozymes in sweet potato root tissue injured by cutting or with black rot. Plant and Cell Physiology, Tokio, v.23, p.1091-1101, 1972.

MARTINEZ, C.; BACCOU, J. –C.; BRESSON, E.; BAISSAC, Y.; DANIEL, J. –F.; JALLOUL, A.; MONTILLET, J. –L.; GEIGER, J. –P.; ASSIGBETSÉ, K.; NICOLE, M. Salicylic acid mediated by the oxidative burst is a key molecule in local and systemic responses of cotton challenged by an avirulent race of Xanthomonas campestris pv malvacerum. Plant Physiology, Bethesda, v. 122, p. 757-766, 2000.

NEPA/UNICAMP. Tabela brasileira de composição de alimentos – TACO. Versão 2. Disponível em: . Acesso em: 04 Nov. 2013.

NUNES, J. M.; PINHATTI, A. V.; POSER, G. L. V.; RECH, S. B.; Influência da elicitação com ácido salicílico na produção de compostos fenólicos em plantas aclimatizadas de hypericum polyanthemum klotzsch ex reichardt.Salão de Iniciação Científica (20: 2008 out. 20-24: Porto Alegre, RS). Livro de resumos. Porto Alegre: UFRGS, 2008.

ROSS, A.F.; Systemic acquired resistance induced by localized virus infections in plants. Virology, San Diego, v.14, p. 340-358, 1961.

RYALS, J.; UKNES, S.; WARD, E. Systemic acquired resistance. Plant Physiology, Bethesda, v. 104, p. 1109-1112, 1994.

SILVA, F.A.S.; AZEVEDO, C.A.V.; Principal Components Analysis in the Software Assistat-Statistical Attendance. In: World congress on computers in agriculture, 7, Reno-NV-USA: American Society of Agricultural and Biological Engineers, 2009.

SPLETZER, M. E.; ENYEDI, A. J. Salicylic acid induces resistance to Alternaria solani in hydroponically grown tomato. Phytopathology, St. Paul, v. 89, p. 722 – 727, 1999. DOI 10.1094/PHYTO.1999.89.9.722.

STADNIK, M. Indução de resistência a oídios. In: CONGRESSO PAULISTA DE FITOPATOLOGIA, Campinas.Anais do Congresso Paulista de Fitopatologia, v. 23, p. 176-181. 2000.

STRACK, D. Phenolic metabolism. In: DEY, P. M., HARBORNE, J. B. (Ed.). Plant Biochemistry. London: Academic Press, p. 387-416. 1997.

VERNOOIJ, B.; UKNES, S.; WARD, E.; RYALS, J. Salicylic acid as a signal molecule in plant-pathogen interactions. Current Opinion in Cell Biology, Danvers, v. 6, p. 275-279, 1994.

VIEIRA, R. S. I. R. Sistema de informação rural. Associação de agricultores da madeira portugal, 2006. Disponível em: http:// w.sirmadeira.org/epages/sir.sf/pt_pt/. Acessado em Outubro de 2013.

WEBER, D.; JONES, E.; BESKOW, T. G.; BARBOSA, M. M.; SAAVEDRA, J.; FACHINELLO, J. C.; Ácido salicílico e refrigeração na conservação de maracujás.Revista Iberoamericana de Tecnología Postcosecha Vol 13(2):123-129. Asociación Iberoamericana de Tecnología Postcosecha, S.C. Hermosillo, México, 2012.ISSN 1665-0204.

YALPANI, N.; SILVERMAN, P.; WILSON, M. A; KLEIER, D. A; RASKIN, I. Salicylic acid is a systemic signal and an inducer of pathogenesis related proteins in virus-infected tobacco. Plant cell, Rockville, v. 3, p. 809-818, 1991.




DOI: https://doi.org/10.5935/PAeT.V7.N3.03