Use of  Agaricus blazei  and  Lentinula edodes  extracts for post-harvest control of gray mold in ‘Itália’ grape

ELISANGELA CLARETE CAMILI; ELIANE APARECIDA BENATO; SÉRGIO FLORENTINO PASCHOLATI; PATRÍCIA CIA

doi:10.5777/paet.v2i2.653

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**Use of Agaricus blazei and Lentinula edodes extracts for post-harvest control of gray mold in ‘Itália’ grape**

ELISANGELA CLARETE CAMILI, ELIANE APARECIDA BENATO, SÉRGIO FLORENTINO PASCHOLATI, PATRÍCIA CIA

Abstract

For protecting ‘Itália’ against Botrytis cinerea in the post harvest it was evaluated in vivo direct and indirect effect of water based extracts of Agaricus blazei and Lentinula edodes treating grape bunches before or after inoculating the pathogen. Two trials were performed: 1) grape bunches were inoculated before and after 4 h they were sprayed with different concentrations of extracts from basidiocarps (0.0; 2.5; 5.0; 10.0; 20.0 or 40.0%); 2) bunches were sprayed or not with A. blazei extracts (5,0%) 24, 48, 72 or 96 h before pathogen inoculation. For inoculation, in each bunch, 10 berries were injured with a ±2 mm deep sting and sprayed with a conidial suspension (±10⁵ conidia mL^-1). After treatment, bunches were kept at 25±1 ºC/80-90% RH and daily evaluated for incidence and severity of rottenness. The in vitro effect of A. blazei e L. edodes extracts for pathogen control were evaluated in order to verify if these agents have a direct effect over mycelia growth and B. cinerea conidia germination. Results show that A. blazei e L edodes extracts did not control rottenness caused by B. cinerea in ‘Italia’ grapes when sprayed before or after fungus inoculation. In the in vitro trials, both basidiocarps extracts stimulated B. cinerea conidia germination at the tested concentrations; regarding mycelia growth, L. edodes had negative, while A. blazei had positive effect.

Keywords

Vitis vinifera; table grapes; Botrytis cinerea

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DOI: https://doi.org/10.5777/paet.v2i2.653

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Applied Research & Agrotechnology

Use of Agaricus blazei and Lentinula edodes extracts for post-harvest control of gray mold in ‘Itália’ grape

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**Use of Agaricus blazei and Lentinula edodes extracts for post-harvest control of gray mold in ‘Itália’ grape**