Differential of expression in Isoenzymas seeds and seedlings F1 of barley

Lilian Madruga Tunes; Pablo Gerzson Badinelli; Maria Alice da Silva de Castro; Antonio Carlos de Souza Albuquerque Barros

doi:10.5777/paet.v2i2.88

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Differential of expression in Isoenzymas seeds and seedlings F1 of barley

Lilian Madruga Tunes, Pablo Gerzson Badinelli, Maria Alice da Silva de Castro, Antonio Carlos de Souza Albuquerque Barros

Abstract

The present work had as objective to evaluate the isoenzimatic patterns of Acid Phosphatase (ACP), Alcohol Dehydrogenase (ADH) and Glutamate Oxalacetate Transaminase (GOT) of two barley cultivars (MN 721 and Scarlett). The seeds were picked in three times with different percentage corresponding to three different steps of their maturation. The seed humidity was reduced to 13% and stored in cold camera. The seeds and the plantlet were analyzed of all cultivars and times. The three isoenzimatic systems analyzed presented variations in the expression, as it was expected when compared between seeds and seedlings. The water content of seeds influences the enzyme pattern of the GOT enzyme. ACP bands were detected in all the treatments evaluated, however, in seeds with lower intensity.

Keywords

Hordeum vulgare L.; electrophoresis; protein extraction; ACP; ADH and GOT

Full Text:

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DOI: https://doi.org/10.5777/paet.v2i2.88

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Applied Research & Agrotechnology

Differential of expression in Isoenzymas seeds and seedlings F1 of barley

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